Step-by-step selection of highly productive by cellulase activity colonies of fungus Trichoderma viride

The paper presents the results of the stepwise selection of highly productive by cellulase activity colonies of the fungus Trichoderma viride ARCIM 13/10 (F-120). To obtain and select T. viride 13/10 colonies, dense nutrient media were used, in which the carbon sources were soluble substrates (2 % sucrose, 2 % sodium carboxy-methyl cellulose) and insoluble, hardly hydrolyzeble substrates (Whatman No. 1 filter paper and cellulose produced by JSC «Mondi Syktyvkar Timber Industry Complex»). Cellulase activity of T. viride 13/10 colonies was assessed by saccharification of filter paper. As a result of the selection of the T. viride 13/10 strain carried out according to the scheme developed by us, it was possible to increase the cellulase activity of the strain in comparison with its initial activity by 6,2-7,0 times. Further selection, especially with the use of mutagenic factors, can further increase the level of cellulase synthesis by the culture of the fungus T. viride .