The properties of Bacillus subtilis strains as amylase producers in the production of sugar-containing fodder additive

The research objective was studying comparative amylolytic activity in various strains of the microorganism Bacillus subtilis when producing sugar-containing fodder additive. The research problems included: 1) studying amilolitic activity at three strains of Bacillus subtilis microorganism: strain № 2-amylolytic, strain № 9-amylolytic and strain № 12-amylolytic; 2) the establishment of the quantity of spores of each strain, giving the maximum amilolitic effect. The results of studying amilolitic activity at various strains of Bacillus subtilis microorganism are given. Developed technology provides the application of one of Bacillus subtilis microorganism strains as a producer of amilolitic enzyme for the purpose of receiving from the starch of sugar-containing fodder additive intended for calves. Amilolitic activity of various strains of Bacillus subtilis microorganism was established: № 2-amylolytic, № 9-amylolytic and № 12-amylolytic, - with the definition of the quantity of spores in the strains giving the maximum amilolitic effect. It was shown that the highest amilolitic activity had been defined at a strain of Bacillus subtilis № 12 - by amylolytic, average - at a strain of Bacillus subtilis № 9-amylolytic and the smallest - at the strain of Bacillus subtilis № 2-amylolytic. The quantity of the spores giving the maximum amilolitic effect at different strains, averaged 125 000 spores/ml. Feeding the calves with sugar-containing fodder additive allows not only to provide their organism with sugars, but also to increase the digestibility of the starch which comes to digestive tract of the calves with vegetable forage. Besides, the strains containing in sacchariferous feed additive microorganisms of Bacillus subtilis possess antagonistic activity in relation to enteropatogennic bacteria, i.e. such additive possesses probiotic properties and therefore its application will not only eliminate deficiency of sugars in a diet, but also prevent gastrointestinal diseases at calves. In addition, the strains of Bacillus subtilis of sugar-containing feed additive have antagonistic activity towards enteropathogenic bacteria, i.e. such additive has probiotic properties, and therefore its use will not only eliminate sugar deficiency in the diet, but also prevent gastrointestinal diseases in calves.