T-cell composition of spleen in rats under acute poisoning with “Extra Confidor”

The results of the study of the spleen T-cell composition in experimental acute poisoning of rats by the pesticide from the group of neonicotinoids were presented. The studies were carried out at theDepartment of Anatomy, Histology, Physiology and Pathological Anatomy of Omsk State Agrarian University. Acute intoxication was performed in animals of experimental group (n = 10) gastrically by means of single administration of a Confidor probe at a dose of 53.6 mg / kg (imidacloprid, 1/10 LD50). To determine qualitative composition of cellular and non-cellular spleen structures, both traditional histological studies and immunohistochemical cell typing with monoclonal antibodies (Daco, USA) were used for revealing the total population of T-lymphocytes (CD3), identifying T-helper cells (CD4), T-suppressors and / or cytotoxic cells (CD8). The nature of immunohistochemical reactions was evaluated visually at points, taking into account staining intensity and the percentage of stained cells...