Test-system for detection of peste des petits ruminants virus genome by reverse transcription real-time PCR

Peste des petits ruminants (PPR) is a highly contagious transboundary infection disease of small ruminants, characterized by fever, anorexia, ocular and nasal discharge, erosions and ulcers in digestive mucosa, diarrhoea and marked leucopoenia with immunosuppression. Because of the complexity of the PPR epizootic situation in neighboring countries (Tajikistan, China, Mongolia, Kazakhstan, Afghanistan) the risk of occurrence of this disease on the territories of Siberian, Ural, Far Eastern, North Caucasian and Southern Federal Districts of Russian Federation is very high. So, the development of molecular-genetic methods for the diagnosis of PPR and methods for stabilizing of biological samples represents scientific relevance. This article presents data on the development of a test system for detecting the PPR virus genome by reverse transcription Real-Time PCR. This technique is based on the amplification of a fragment (148 bp) of hemagglutinin gene of PPR virus using the original oligonucleotide primers and fluorescent-labeled hybridization probe...