The influence of the constant illumination on the ultrastructure of rat's hepatocytes

The influence of the constant illumination on the ultrastructure of rat's hepatocytes

Автор: Areshidze David A., Kozlova Mariya A., Chernikov Valery P., Kondashevskaya Marina V.

Журнал: Морфологические ведомости @morpholetter

Рубрика: Оригинальные исследования

Статья в выпуске: 1 т.31, 2023 года.

Бесплатный доступ

The disorganization of natural biorhythms in the modern world is mostly attributed to the violation of circadian rhythms due to light pollution. Urbanization is directly interconnected with an excess of artificial lighting. As a one-time phenomenon, light pollution leads to a reversible shift in circadian rhythms, but in the case of constant influence, it leads to the development of desynchronosis. It is known that light pollution contributes to the development of nonalcoholic fatty liver disease, primary biliary cirrhosis, and metabolic disorders. The study aimed to investigate the changes in micro-morphometric parameters and ultrastructure of hepatocytes of Wistar rats under the influence of normal lighting and constant lighting exposure. This study was conducted on 120 outbred stock male Wistar rats at an age of 6 months, with a body weight of 350 g. The rats were divided into 2 equal groups. The control group included 60 rats, kept in standard laboratory conditions under a normal cyclical «light-dark» lighting regime (10:14, 10 hours of light - from 8:00 to 18:00, 14 hours of darkness - from 18:00 to 8:00) within 3 weeks. The experimental group included 60 rats kept in standard laboratory conditions under constant lighting within 3 weeks. To obtain the results, histological, micro morphometric methods and transmission electronic microscopy were used. The revealed changes of the hepatocytes under the influence of constant lighting indicate that a violation of the illumination regime is a potent factor causing damage and structural changes in the liver. Understanding the mechanisms underlying the liver's response to circadian rhythm disruption and associated damage is important to form patient-specific recommendations on lifestyle and behavioral regimens.

Еще

Hepatocyte, liver, micromorphometry, constant lightning, desynchronosis

Короткий адрес: https://sciup.org/143180239

IDR: 143180239   |   DOI: 10.20340/mv-mn.2023.31(1).758

Список литературы The influence of the constant illumination on the ultrastructure of rat's hepatocytes

  • VitaternaMH, Takahashi JS, TurekFW. Overview of circadian rhythms. Alcohol Res Health. 2001;25(2):85-93
  • Boyce PR. Human factors in lighting. Third Edition. Boca Raton-London-New York: Crc Press, 2014.- 703pp
  • Jasser SA, Blask DE, Brainard GC. Light during darkness and cancer: relationships in circadian photoreception and tumor biology. Canc Caus Con. 2006;17(4):515-523. DOI: 10.1007/s10552-005-9013-6
  • Fazakas J, Mändli T, Ther G et al. Evaluation of liver function for hepatic resection. Transplant Proc. 2006;38(3):798-800. DOI: 10.1016/j.transproceed.2006.01.048
  • Parkinson A, Ogilvie BW. Biotransformation of xenobiotics. Casarett and Doull's toxicology: the basic science of poisons. 2008;7:161-304
  • Kim P, Oster H, Lehnert H et al. Coupling the Circadian Clock to Homeostasis: The Role of Period in Timing Physiology. Endocr Rev. 2019;40(1):66-95. DOI: 10.1210/er.2018-00049
  • Shi D, Chen J, Wang J et al. Circadian Clock Genes in the Metabolism of Non-alcoholic Fatty Liver Disease. Front Physiol. 2019;10:423. DOI: 10.3389/fphys.2019.00423
  • Mukherji A, Bailey SM, Staels B, Baumert TF. The circadian clock and liver function in health and disease. J Hepatol. 2019;71(1):200-211. DOI: 10.1016/j.jhep. 2019.03.020
  • Hastings MH, Maywood ES, Brancaccio M. Generation of circadian rhythms in the suprachiasmatic nucleus. Nat Rev Neurosci. 2018;19(8):453-469. DOI: 10.1038/s41583-018-0026-z
  • Balsalobre A, Brown SA, Marcacci L et al. Resetting of circadian time in peripheral tissues by glucocorticoid signaling. Science. 2000;289(5488):2344-2347. DOI: 10.1126/science.289.5488.2344
  • Guerrero-Vargas NN, Guzmân-Ruiz M, Fuentes R et al. Shift Work in Rats Results in Increased Inflammatory Response after Lipopolysaccha-ride Administration: A Role for Food Consumption. J Biol Rhythms. 2015;30(4):318-330. DOI: 10.1177/0748730415586482
  • Guilding C, Hughes AT, Brown TM et al. A riot of rhythms: neuronal and glial circadian oscillators in the mediobasal hypothalamus. Mol Brain. 2009;2:28. DOI: 10.1186/1756-6606-2-28
  • Brown SA, Zumbrunn G, Fleury-Olela F et al. Rhythms of mammalian body temperature can sustain peripheral circadian clocks. Curr Biol. 2002;12(18):1574-1583. DOI: 10.1016/s0960-9822(02)01145-4
  • Westerterp-Plantenga MS. Challenging energy balance - during sensitivity to food reward and modulatory factors implying a risk for overweight - during body weight management including dietary restraint and medium-high protein diets. Physiol Behav. 2020;221:112879. DOI: 10.1016/j .physbeh.2020.112879
  • Leung JM, Martinez ME. Circadian Rhythms in Environmental Health Sciences. Curr Environ Health Rep. 2020;7(3):272-281. DOI: 10.1007/s40572-020-00285-2
  • Aho V, Ollila HM, Kronholm E et al. Prolonged sleep restriction induces changes in pathways involved in cholesterol metabolism and inflammatory responses. Sci Rep. 2016;6:24828. DOI: 10.1038/srep24828
  • Poggiogalle E, Jamshed H, Peterson CM. Circadian regulation of glucose, lipid, and energy metabolism in humans. Metabolism. 2018;84:11-27. DOI: 10.1016/j.metabol.2017.11.017
  • Mota MC, Silva CM, Balieiro LCT et al. Social jetlag and metabolic control in non-communicable chronic diseases: a study addressing different obesity statuses. Sci Rep. 2017;7(1):6358. DOI: 10.1038/s41598-017-06723-w
  • Anisimov VN. Light desynchronosis and health. Light & Engineering (2019);27.3:14-25
  • Masri S, Sassone-Corsi P. The emerging link between cancer, metabolism, and circadian rhythms. Nat Med. 2018;24(12):1795-1803. DOI: 10.1038/s41591-018-0271-8
  • Verlande A, Masri S. Circadian Clocks and Cancer: Timekeeping Governs Cellular Metabolism. Trends Endocrinol Metab. 2019;30(7):445-458. DOI: 10.1016/j.tem.2019.05.001
  • Wei L, Yue F, Xing L et al. Constant light exposure alters gut microbiota and promotes the progression of Steatohepatitis in high fat diet rats. Front Microbiol. 2020;11:1975. DOI: 10.3389/fmicb.2020.01975
  • Heathcote J. Treatment of primary biliary cirrhosis. J Gastroenterol Hepatol. 1996;11(7): 605-609. DOI: 10.1111/j.1440-1746.1996.tb00300.x
  • van den Heiligenberg S, Deprés-Brummer P, Barbason H et al. The tumor promoting effect of constant light exposure on diethylnitrosamine-induced hepatocarcinogenesis in rats. Life Sci. 1999;64(26):2523-2534. DOI: 10.1016/s0024-3205(99)00210-6
  • Walker WH, Bumgarner JR, Walton JC et al. Light Pollution and Cancer. Int J Mol Sci. 2020;21(24):9360. DOI: 10.3390/ijms21249360
  • Nelson RJ, Chbeir S. Dark matters: effects oflight at night on metabolism. Proc Nutr Soc. 2018;77(3):223-229. DOI: 10.1017/S0029665118000198
  • Li W, Li L, Hui L. Cell Plasticity in Liver Regeneration. Trends Cell Biol. 2020;30(4):329-338. DOI: 10.1016/j.tcb.2020.01.007
  • Junatas KL, Tonar Z, Kubikovâ T, Liska V, Pâlek R, Mik P, Krâlickovâ M, Witter K. Stereological analysis of size and density of hepatocytes in the porcine liver. J Anat. 2017;230(4):575-588. DOI: 10.1111/joa.12585
  • Smitha T, Sharada P, Girish H. Morphometry of the basal cell layer of oral leukoplakia and oral squamous cell carcinoma using computer-aided image analysis. J. Oral Maxillofac Pathol. 2011;15:26-33
  • Häussinger D, Graf D, Weiergräber OH. Glutamine and cell signaling in liver. J Nutr. 2001;131(9 Suppl):2509S-14S; discussion 2523S-4S. DOI: 10.1093/jn/131.9.2509S
  • Bardeck N, Paluschinski M, Castoldi M et al. Liver cell swelling leads to upregulation of miR-141-3p in perfused rat liver and primary rat hepatocytes. Zeitschrift für Gastroenterologie. 2021;59(01):1-16. DOI: 10.1055/s-0040-1721965
  • Miyaoka Y, Ebato K, Kato H et al. Hypertrophy and unconventional cell division of hepatocytes underlie liver regeneration. Curr Biol. 2012;22(13):1166-1175. DOI: 10.1016/j.cub.2012.05.016
  • Lazzeri E, Angelotti ML, Conte C et al. Surviving Acute Organ Failure: Cell Polyploidization and Progenitor Proliferation. Trends Mol Med. 2019;25(5):366-381. DOI: 10.1016/j.molmed.2019.02.006
  • Nagy P, Teramoto T, Factor VM et al. Reconstitution of liver mass via cellular hypertrophy in the rat. Hepatology. 2001;33(2):339-45. DOI: 10.1053/jhep.2001.21326
  • Miyaoka Y, Miyajima A. To divide or not to divide: revisiting liver regeneration. Cell Div. 2013;8(1):8. DOI: 10.1186/1747-1028-8-8
  • Anderson WR, Zieve L, Lindblad S. Ultrastructural study of hepatic regeneration following one-lobe, two-lobe, and subtotal hepatectomy in the rat. Exp Pathol. 1990;38(1):61-72. DOI: 10.1016/s0232-1513(11)80200-8
  • Khraiwesh H, Löpez-Dominguez JA, Lôpez-Lluch G et al. Alterations of ultrastructural and fission/fusion markers in hepatocyte mitochondria from mice following calorie restriction with different dietary fats. J Gerontol A Biol Sci Med Sci. 2013;68(9):1023-1034. DOI: 10.1093/gerona/glt006
  • Mathes AM. Hepatoprotective actions of melatonin: possible mediation by melatonin receptors. World J Gastroenterol. 2010;16(48): 6087-6097. DOI: 10.3748/wjg.v16.i48.6087
  • Chojnacki C, Walecka-Kapica E, Romanowski M et al. Protective role of melatonin in liver damage. Curr Pharm Des. 2014;20(30):4828-4833. DOI: 10.2174/1381612819666131119102155
  • Esteban-Zubero E, Alatorre-Jiménez MA, Löpez-Pingarrön L et al. Melatonin's role in preventing toxin-related and sepsis-mediated hepatic damage: A review. Pharmacol Res. 2016;105:108-120. DOI: 10.1016/j.phrs.2016.01.018
  • Abbasoglu O, Berker M, Ayhan A rt al. I. The effect of the pineal gland on liver regeneration in rats. Journal of Hepatology. 1995;23(5):578-581. DOI:10.1016/0168-8278(95)80065-4
  • Liang R, Nickkholgh A, Hoffmann K et al. Melatonin protects from hepatic reperfusion injury through inhibition of IKK and JNK pathways and modification of cell proliferation. J Pineal Res. 2009;46(1):8-14. DOI: 10.1111/j.1600-079X.2008.00596.x
  • Kobayashi T, Saito Y, Ohtake Y et al. Effect of aging on norepinephrine-related proliferative response in primary cultured periportal and perivenous hepatocytes. Am J Physiol Gastrointest Liver Physiol. 2012;303(7):G861-869. DOI: 10.1152/ajpgi.00081.2012
  • Wilkinson PD, Duncan AW. Differential Roles for Diploid and Polyploid Hepatocytes in Acute and Chronic Liver Injury. Semin Liver Dis. 2021;41(1):42-49. DOI: 10.1055/s-0040-1719175
  • Yanko R. The combined influence of the intermittent normobaric hypoxia and melatonin on morphofunctional activity of the rat's liver parenchyma. Bulletin of Taras Shevchenko National University of Kyiv -Problems of Physiological Functions Regulation. 2018;25(2):36-40
Еще
Статья научная
QR-код для установки приложения SciUp Наведите камеру и скачайте бесплатное приложение SciUp