The molecular detection of the Anaplasma ovis pathogens of the serological samples in small ruminants and ixodid ticks in Azerbaijan

The molecular detection of the Anaplasma ovis pathogens of the serological samples in small ruminants and ixodid ticks in Azerbaijan

Автор: Azizova A.

Журнал: Бюллетень науки и практики @bulletennauki

Рубрика: Сельскохозяйственные науки

Статья в выпуске: 12 т.9, 2023 года.

Бесплатный доступ

A total of 704 blood samples (561 sheep, 143 goats) were examined for the Anaplasma ovis infection during a 1-year period. PCR and ELISA were used for the detection of the A. ovis antibodies. PCR assay identified A. ovis in 20 (8.1%) sheep and 15 (6.09%) goats. Using ELISA assay, 8.53% (31) were positive (21 sheep, 10 goats). A total of 246 blood smears were examined for the presence of intra-erythrocytic inclusions using Giemsa stain. Among the collected specimens, 60 were found positive with an overall prevalence of 24.3%. Among the 60 positive animals, 26 (43.3%) were sheep and 34 (56.7%) were goat. In the peripheral blood samples, the other piroplasmids - Babesia ovis, Theileria ovis, Th. recondita - were followed in an associative form. The ticks were collected, and the species composition was appointed in order to determine the ticks parasitized and have a pathogenic lifestyle in small ruminants. The intensive infection with the ticks of 2 genera - Rhipicephalus and Hyalomma was followed in small ruminants. It was determined that 45.8% of sheep and 35.1% of goats were infected intensively with the ticks of the Hyalomma genus. 110 samples prepared from the ticks of the Hyalomma genus were tested from the PCR test according to the Anaplasma ovis pathogen. 45 samples (40.9%) were assessed positively that 21 samples of them belonged to sheep and 24 of them to goats. 80 samples prepared from the internal organs of the ticks were examined according to the A. ovis parasite and the obtained results were analyzed. In 5 out of 35 samples which detected the parasites, A. ovis was followed, and in 30 samples, the associative parasites: Th. ovis, B. ovis piroplasmids. The Rickettsia and Coxiella pathogens were also detected in the samples.

Еще

Molecular identification, polymerase chain reaction, elisa, anaplasma, ixodidae, small ruminants

Короткий адрес: https://sciup.org/14129283

IDR: 14129283   |   DOI: 10.33619/2414-2948/97/21

Список литературы The molecular detection of the Anaplasma ovis pathogens of the serological samples in small ruminants and ixodid ticks in Azerbaijan

  • Azizova, A. A. (2022). The associative invasions - the helminths, primary intestinal parasites, piroplasmids of the small ruminants in Azerbaijan. In 7th International New York Conference on evolvingtrends in interdisciplinary research and practices Proceedings book. New York, 97-103.
  • Jonsson, N. N., Bock, R. E., & Jorgensen, W. K. (2008). Productivity and health effects of anaplasmosis and babesiosis on Bos indicus cattle and their crosses, and the effects of differing intensity of tick control in Australia. Veterinary parasitology, 155(1-2), 1-9. https://doi.org/10.1016/j.vetpar.2008.03.022
  • Ybanez, A. P., Sashika, M., & Inokuma, H. (2014). The phylogenetic position of Anaplasma bovis and inferences on the phylogeny of the genus Anaplasma. Journal of Veterinary Medical Science, 76(2), 307-312. https://doi.org/10.1292/jvms.13-0411
  • Hornok, S., Elek, V., de La Fuente, J., Naranjo, V., Farkas, R., Majoros, G., & Földvári, G. (2007). First serological and molecular evidence on the endemicity of Anaplasma ovis and A. marginale in Hungary. Veterinary microbiology, 122(3-4), 316-322. https://doi.org/10.1016/j.vetmic.2007.01.024
  • Renneker, S., Abdo, J., Salih, D. E. A., Karagenç, T., Bilgiç, H., Torina, A., ... & Seitzer, U. (2013). Can Anaplasma ovis in small ruminants be neglected any longer?. Transboundary and emerging diseases, 60, 105-112. https://doi.org/10.1111/tbed.12149
  • Azizova, A. A., & Rustamova, S. I. (2021). The distribution dynamics of the piroplasmid (Piroplasmida) and transmitting ticks in the agricultural animals. In Fundamental and applied scientific investigations in zoology: Actual problems, achievements and innovations. Baku, 23-27.
  • Azizova A. A. (2022). The morphological parameters of the blood of sheep infected with the associative invasion. Endless Light in Science, (3-3), 350-357. https://doi.org/10.24412/2709-1201-2022-2022-350-357
  • Bradway, D. S., de Echaide, S. T., Knowles, D. P., Hennager, S. G., & McElwain, T. F. (2001). Sensitivity and specificity of the complement fixation test for detection of cattle persistently infected with Anaplasma marginale. Journal of veterinary diagnostic investigation, 13(1), 79-81. https://doi.org/10.1177/10406387010130011
  • Mason, K. L., Gonzalez, M. V., Chung, C., Mousel, M. R., White, S. N., Taylor, J. B., & Scoles, G. A. (2017). Validation of an improved Anaplasma antibody competitive ELISA for detection of Anaplasma ovis antibody in domestic sheep. Journal of Veterinary Diagnostic Investigation, 29(5), 763-766. https://doi.org/10.1177/104063871770949
  • Ekici, O. D., & Sevinc, F. (2011). Comparison of cELISA and IFA tests in the serodiagnosis of anaplasmosis in cattle. African Journal of Microbiology Research, 5(10), 1188-1191.
  • Dumler, J. S., & Brouqui, P. (2004). Molecular diagnosis of human granulocytic anaplasmosis. Expert Review of Molecular Diagnostics, 4(4), 559-569.https://doi.org/10.1586/14737159.4.4.559
  • Torina, A., Agnone, A., Blanda, V., Alongi, A., D’Agostino, R., Caracappa, S., ... & de la Fuente, J. (2012). Development and validation of two PCR tests for the detection of and differentiation between Anaplasma ovis and Anaplasma marginale. Ticks and tick-borne diseases, 3(5-6), 283-287. https://doi.org/10.1016/j.ttbdis.2012.10.033
  • Bowman, D. D. (2006). Successful and currently ongoing parasite eradication programs. Veterinary Parasitology, 139(4), 293-307. https://doi.org/10.1016/j.vetpar.2006.04.020
  • Dumanli, N. (2016). Şanlıurfa yöresinde koyun ve keçilerde Anaplasma phagocytophilum’un moleküler yöntemlerle araştırılması.
  • Noaman, V., & Sazmand, A. (2022). Anaplasma ovis infection in sheep from Iran: molecular prevalence, associated risk factors, and spatial clustering. Tropical Animal Health and Production, 54(1), 6. https://doi.org/10.1007/s11250-021-03007-4
  • Johan S., Bakken J., Stephen Dumler.Human Granulocytic Anaplasmosis. Infect Dis Clin North Am. 2015 Jun; 29(2): 341–355.doi: 10.1016/j.idc.2015.02.007
  • Bakken, J. S., & Dumler, J. S. (2015). Human granulocytic anaplasmosis. Infectious Disease Clinics, 29(2), 341-355. https://doi.org/10.1016/j.idc.2015.02.007
  • Thomas, R. J., Dumler, J. S., & Carlyon, J. A. (2009). Current management of human granulocytic anaplasmosis, human monocytic ehrlichiosis and Ehrlichia ewingii ehrlichiosis. Expert review of anti-infective therapy, 7(6), 709-722. https://doi.org/10.1586/eri.09.44
  • De Waal, T. (2012). Advances in diagnosis of protozoan diseases. Veterinary Parasitology, 189(1), 65-74. https://doi.org/10.1016/j.vetpar.2012.03.033
  • Silva, V. M., Araújo, F. R., Madruga, C. R., Soares, C. O., Kessler, R. H., Almeida, M. A., ... & Torres Júnior, R. A. (2006). Comparison between indirect enzyme-linked immunosorbent assays for Anaplasma marginale antibodies with recombinant major surface protein 5 and initial body antigens. Memórias do Instituto Oswaldo Cruz, 101, 511-516. https://doi.org/10.1590/S0074-02762006000500005
  • Zhang, X. C., Zhang, L. X., Li, W. H., Wang, S. W., Sun, Y. L., Wang, Y. Y., ... & Zhang, L. J. (2012). Ehrlichiosis and zoonotic anaplasmosis in suburban areas of Beijing, China. Vector-Borne and Zoonotic Diseases, 12(11), 932-937. https://doi.org/10.1089/vbz.2012.0961
  • Niu, Q., Luo, J., Guan, G., Ma, M., Liu, Z., Liu, A., ... & Yin, H. (2009). Detection and differentiation of ovine Theileria and Babesia by reverse line blotting in China. Parasitology research, 104, 1417-1423. https://doi.org/10.1007/s00436-009-1344-x
  • Dey, A., & Singh, S. (2009). Progress of science from microscopy to microarrays (Part 1): Diagnosis of parasitic diseases. Journal of Laboratory Physicians, 1(01), 002-006. https://doi.org/10.4103/0974-2727.54800
Еще
Статья научная
QR-код для установки приложения SciUp Наведите камеру и скачайте бесплатное приложение SciUp