The responses of ascorbate - glutathione cycle enzymes in seedlings of Pancratium maritimum L. under drought treatments

The responses of ascorbate - glutathione cycle enzymes in seedlings of Pancratium maritimum L. under drought treatments

Автор: Seckin Dinler Burcu, Aksoy Merve

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 2 т.9, 2013 года.

Бесплатный доступ

In this study, physiological and biochemical responses of ( Pancratium maritimum L.), desert plant which is very widespread on coastal sand dunes to drought were determined. Therefore 28 days (d) old plants were drought stressed by withholding water for 5 and 10 days. The changes in relative growth rate (RGR), relative water content (RWC) lipid peroxidation, and ascorbate-glutathione cycle enzymes activity ((ascorbate peroxidase (APX, EC 1.11.1.11), glutathione reductase (GR, EC 1.6.4.2) dehydroascorbate reductase (DHAR, EC 1.8.5.1) and monodehydroascorbate reductase (MDAR, EC 1.6.5.4)) were investigated. Relative growth rate, relative water content were both decreased on the 5 and 10d of stress treatment while it was higher on the 10d. MDA content increased on the 10d while it did not change on the 5d. On the other hand, activities of APX, GR, MDAR and DHAR increased on the 5d but were not change on the 10d. These results suggest that ascorbate – glutathione cycle enzymes were efficient to prevent from oxidative damage under short term of drought stress in ( Pancratium maritimum L.) plants.

Еще

Pancratium maritimum, drought stress, ascorbate- glutathione cycle

Короткий адрес: https://sciup.org/14323731

IDR: 14323731

Список литературы The responses of ascorbate - glutathione cycle enzymes in seedlings of Pancratium maritimum L. under drought treatments

  • Abogadallah, G.M. (2011). Differential regulation of photorespiratory gene expression by moderate and severe salt and drought stress in relation to oxidative stress. Plant Sci. 180: 540-547.
  • Alscher, R.G., Ertuk, N. Heath, L.S. (2002). Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J. Exp. Bot. 372:1331-1341.
  • Bian, S. Jiang, Y. (2009). Reactive oxygen species, antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery. Science Horticulture-Amsterdam. 120: 264-270.
  • Cruz de Carvalho, M.H. (2008). Drought stress and reactive oxygen species. Plant Signal Behaviour. 3: 156-165.
  • Dothan, N.F. (1986). Flora Palaestina, Israel Academy of Sciences and Humanities, Jerusalem, Israel.
  • Foyer, C.H., Halliwell, B. (1976). The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta. 133:21-25.
  • França, M.G.C., Thi, A.T.P., Pimentel, C., Rossiello, R.P.R., Zuily-Fodil, Laffray, Y.D. (2000). Differences in growth and water relations among Phaseolus vulgaris cultivars in response to induced drought stress. Environ. and Exp. Bot. 43: 227-237.
  • Grassi, F., Cazzanigal, E., Minuto, L., Peccenini, S., Barberis, G., Basso, B.G. (2005). Evaluation of biodiversity and conversation strategies in Pancratium maritimum L. for the Northern Tyrrhenian Sea. Biodiversity and Conservation. 14: 2159-2169.
  • Guo, Z., Ou, W., Lu, S., Zhong, Q. (2006) Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol. Biochem. 44: 828-36.
  • Halliwell, B., Gutteridge, J.M.C. (1989). Protection against oxidants in biological systems: the super oxide theory of oxygen toxicity.In: Halliwell, B., Gutteridge, J.M.C. (Eds.), Free Radical Biol. Med. 86-123, Clarendon Press, Oxford.
  • Hernandez, J.A., Jimenez, A., Mullineaux, P.M., Sevilla, F. (2000). Tolerance of pea (Pisum sativum L) to long-term salt stress is associated with induction of antioxidant defences. Plant Cell and Environ. 23: 853-862.
  • Jaleel, C.A., Manivannan, P., Lakshmanan, G.M.A., Gomathinayagam, M., Panneerselvam, R. (2008). Alterations in morphological parameters and photosynthetic pigment responses of Catharanthus roseus under soil water deficits. Colloids Surface B. 61: 298-303.
  • Jiang, M., Zhang, J. (2002). Water stress induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and upregulates the acitivites of antioxidant enzymes in maize leaves. J. Exp. Bot. 53: 2401-10.
  • Khedr, A.H., Abbas, M.A., Wahid, A.A., Quick, W.P., Abogadallah, G.B. (2003). Proline induces the expression of salt -stress-responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt stress. J. Exp.Bot. 54: 2253-2256.
  • Kılınç, M., Karaer, F. (1995). Sinop Yarımadasının Vejetasyonu. Turkish Journal of Bot. 19: 107-124.
  • Krivosheeva, A., Tao, D.L., Ottander, C., Wingsle, G., Dube, S.L., Oquist, G. (1996). Cold acclimation and photoinhibition of photosynthesis in Scots pine. Planta. 200: 296-305.
  • Lascano, H.R., Antonicelli, G.E., Luna, C.M., Melchiorre, M.N., Gomez, L.D., Raca, R.W, Trippi, V.S., Casano, L.M. (2001). Antioxidant system response of different wheat cultivars under drought: Field and in vitro studies. Australian J. of Plant Physiol. 28: 1095-102.
  • Madhava, R.K.V., Sresty, T.V.S. (2000). Antioxidative parameters in the seedlings of pigeon pea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses. Plant Sci. 157: 113-128.
  • Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7: 405-410.
  • Mittler, R., Merquiol, E., Hallak-Herr, E., Rachmilevitch, S., Kaplan, Cohen, A.M. (2001). Living under a dormant canopy: a molecular acclimation mechanism of the desert plant Retama raetam. Plant Journal. 25: 407 -416.
  • Nakano, Y., Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22: 867-880.
  • Noctor, G., Veljovic-Jovanovic,S., Driscoll, S., Novitskaya, L., Foyer, C.H. (2002). Drought and oxidative load in leaves of C3 plants: a predominant role for photorespiration? Annual Bot-London 89: 841-850.
  • Pastori, G.M., Foyer, C.H. (2002). Common components, networks and pathways of cross-tolerance to stress. The central role of redox and abscisic acid-mediated controls. Plant Physiol. 129: 460-468.
  • Ping, B.L., Fang-Gong, S.U.I, Ti-Da, G.E., Zhao-Hui, S., Yin-Yan, L.U., Guang-Sheng, Z. (2006). Effect of Soil Drought Stress on Leaf Water Status, Membrane Permability and Enzymatic Antioxidant System of Maize. Pedosphere. 16: 326-332.
  • Ramachandra, R., Wiswanatha Chaitanya, Vivekanandan, K. M. (2004). Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiol. 161: 1189-1202.
  • Ratnayaka, H.H., Molin, W.T., Steriling, T.M. (2003). Physiological and antioxidant responses of cotton and spurred anoda under interference and mild drought. Journal of Experimental Bot. 54: 2293-2305.
  • Sanaa, A., Ben Fadhel, N.B. (2010). Genetic diversity in mainland and island populations of the endangered Pancratium maritimum L. (Amaryllidaceae) in Tunisia. Science Hortica-England 125: 740-747.
  • Schubert, S., Serraj, R., Plies-Balzer, E., Mengel, K. (1995). Effect of Drought Stress on Growth, Sugar Concentrations and Amino Acid Accumulation in N2-Fixing Alfafa (Medicago sativa). Journal of Plant Physiol. 146: 541-546.
  • Sgherri, C.L.M., Navari-Izzo, F. (1995). Sunflower seedlings subjected to increasing water deficit stress: Oxidative stress and defense mechanism. Physiologia Plantarum. 93: 25-30.
  • Sharma, P., Dubey, R.S. (2005). Drought induces oxidative stress and enhances the acitivites of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation. 46: 209-221.
  • Smart, R.E., Bingham, G.E. (1974). Rapid estimates of relative water content. Plant Physiol. 53: 258-260.
  • Venus, J.C., Causton, D.R. (1979). Plant growth analyses: a re-examination of the methods of calculation of relative growth rate and net assimilation rates without using fitted functions. Annual Bot. 43: 633-638.
  • Yu-Hua, M., Feng-Whang, M., Yong-Hong, W., Jun-Ke, Z. (2011). The responses of the enzymes related with ascorbate-glutathione cycle during drought stress in apple leaves. Acta Physiologia Plantarum. 33: 1, 173-180.
  • Zhao, C.X., Guo, L.Y., Jaleel, C.A., Shao, H.B., Yang, H.B.(2008). Prospects for dissecting plant-adaptive molecular mechanisms to improve wheat cultivars in drought environments. Comp Rend Soc Biol. 331: 579-586.
Еще
Статья научная
QR-код для установки приложения SciUp Наведите камеру и скачайте бесплатное приложение SciUp