The role of sugar-related regulation in the light-dependent alterations of Arabidopsis glutamate dehydrogenase genes expression
Автор: Garnik E.Yu., Belkov V.I., Tarasenko V.I., Konstantinov Yu.M.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 4 т.10, 2014 года.
Бесплатный доступ
Expression of gdh1 and gdh2 genes of Arabidopsis thaliana increases in the dark and decreases in the light. The reason of such alteration seems to be a glucose rising in photosynthetic cell in the light, but this hypothesis needs to be confirmed. In this work we investigate the role of glucose and hexokinase 1 in the light-dependent regulation of the gdh1 and gdh2 expression. A comparison of expression profiles of apl3, gdh1, gdh2 genes in presenсe of exogenous sucrose in the dark and in the light has demonstrated that sugar-related repression of gdh1 and gdh2 genes is insufficient to provide the high decrease of their transcripts in the light. Using Arabidopsis mutant gin2-1 with a defect in hxk1 gene we demonstrated that such a decrease is not depended on the regulatory function of hexokinase 1. We presume that light- dependent alterations of gdh1 and gdh2 expression are mediated by some chloroplast-to-nucleus regulatory signals.
Arabidopsis thaliana, arabidopsis mutants, glutamate dehydrogenase, hexokinase 1, sugar sensing and signaling
Короткий адрес: https://sciup.org/14323912
IDR: 14323912
Список литературы The role of sugar-related regulation in the light-dependent alterations of Arabidopsis glutamate dehydrogenase genes expression
- Garnik E.Yu., Belkov V.I., Tarasenko V.I., Potapova T.V., Korzun M.A., Konstantinov Yu.M. (2013) The expression of Arabidopsis glutamate dehydrogenase gene gdh2 is induced under the influence of tetrapyrrole synthesis inhibitor norflurazon. J. Stress Physiol. Biochem., 4, 299-309
- Hausler R., Heinrich L., Schmitz J., Flugge U.-I. (2014) How sugars might coordinate chloroplast and nuclear gene expression during acclimation to high light intensities. Molecular Plant, 7, 1121-1137
- Hong S.M., Bahn S.C., Lyu A., Jung H.S., Ahn J.H. (2010) Identification and testing of superior reference genes for starting pool of transcript normalization in Arabidopsis. Plant Cell Physiol., 51, 1694-1706
- Miyashita Y., Good A.G. (2008) NAD(H)-dependent glutamate dehydrogenase is essential for the survival of Arabidopsis thaliana during dark-induced carbon starvation. J. Exp. Bot., 59, 667-680
- Moore B., Zhou L., Rolland F., Hall Q., Cheng W.H., Liu Y.X., Hwang I., Jones T., Sheen J. (2003) Role of the Arabidopsis glucose sensor HXK1 in nutrient, light, and hormonal signaling. Science, 300(5617), 332-336
- Murashige T., Skoog F. (1962) Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473-497
- Price J., Laxmi A., St. Martin S.K., Jang J.-C. (2004) Global transcription profiling reveals multipler sugar signal transduction mechanisms in Arabidopsis. Plant Cell, 16, 2128-2150
- Ramon M., Rolland F., Sheen J. (2008) Sugar sensing and signaling. The Arabidopsis Book, 6:e0117 ( ) DOI: 10.1199/tab.0117
- Rook F., Handingham A., Li Y., Bevan M.W. (2006) Sugar and ABA response pathways and the control of gene expression. Plant Cell Environ., 29, 426-434
- Tarasenko V.I., Garnik E.Yu., Shmakov V.N., Konstantinov Yu.M. (2009) Induction of Arabidopsis gdh2 gene expression during changes in redox state of the mitochondrial respiratory chain. Biochemistry (Mosc.), 74(1), 47-53
- Turano F., Thakkar S., Fang T., Weisemann J. (1997) Characterization and expression of NAD(H)-dependent glutamate dehydrogenase genes in Arabidopsis. Plant Physiol., 113, 1329-1341
