Solid-phase enzyme-linked immunosorbent assay (direct option) for detecting toxigenic vibrio cholerae serogroups О1 and О139

As a result of the studies, the method of direct solid-phase enzyme immunoassay (ELISA test) was optimized for detecting toxigenic strains of Vibrio cholerae O1 and O139 serogroups based on monoclonal and polyclonal antitoxic immunoglobulin peroxidase conjugates. The study detected the following optimal parameters for the analysis: sensitization of a tablet by toxicodermia drugs diluted in phosphate-saline buffer (PBS) pH 7.4, up to titers of 1:2-1:4, their adsorption on the solid phase of the tablet for 2 hours at 37° C, blocking of free binding sites by the 1% solution of bovine serum albumin (BSA) for 30 minutes (at 37°C); contact time of the investigated sample with mono- or polyclonal conjugates peroxidase - 30 minutes (at 37°C). Tests on homologous and heterologous strains showed a high specificity of the enzyme immunoassay and the possibility of its use for detecting toxigenic Vibrio cholerae.