Participation of 3-benzylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione in chemical communication among microorganisms

The influence of bifidobacterium B. bifidum 237 on E. coli 12 was studied by the microbiological methods. The inhibitory effect of B. bifidum 237, previously treated with supernatants of E. coli 12, on the persistent properties of the E. coli was found. 3-Benzylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione was detected in supernatants of bacteria by GCMS method. Its quantity correlated with the effects of the mutual influence of association members in a series of experiments. The fragmentation properties of 3-benzylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione were discussed.