Nitrogen oxide role in manifestation of respiratory effects of increased exogenous IL-1B level in blood-vascular system

The purpose of the study is to identify the role of nitrergic mechanisms in the ability of the pro-inflammatory cytokine IL-1§ to influence the respiration pattern and hypoxic ventilation response. Materials and Methods. The experiments were performed on 42 anesthetized rats. To conduct an inhibitory analysis of the nitric oxide role in the manifestation of IL-1fi respiratory effects, the authors used a non-selective inhibitor of NO-synthases of Nitro-L-arginine-methyl ether (L-NAME), and a highly specific inhibitor of inducible nitric oxide synthase, aminoguanidine bicarbonate. The hypoxic ventilation response was evaluated by a rebreathing method with a hypoxic gas mixture before and after intravenous administration of human recombinant IL-1§. Pneumatic tachometry was used to register the parameters of external respiration. Results. Intravenous administration of IL-1§ has an activating effect on respiration and causes an increase in tidal volume by 36±5.2 %, minute respiration volume by 23±3...