Simplification of trypsin digest of proteom E. coli in searching for RNA-polymerase peptides by mass-spectrometry with electrospray ionization (ES-TOF-Ms)

Simplification of trypsin proteom E. coli in searching for RNA-polymerase peptides has been achieved by using gel-penetrating chromatography. As a result of analyzing the fractions obtained in the process of chromatography by the method of time-of-flight mass spectrometry with electrospray ionization, peptides were identified, the presence of which is the evidence of the presence of RNA-polymerase in the initial total protein.