CD45+-, CD-3+-cell level in the liver during physical activity of different degrees and after melodinum administration

Objective: to evaluate the proportion of leukocytes (CD45+ cells) and T-lymphocytes (CD3+ cells) in the liver during physical activity of varying degrees with and without meldonium. Material and methods. The experiments were conducted on male Wistar rats, which performed physical activity of varying intensity. Meldonium was added to the diet of experimental animals at a rate of 100–120 mg/kg of weight during water loading. After the end of the experiment, liver samples were taken, sections were prepared, stained with hematoxylin and eosin, and an immunohistochemical (IHC) reaction was performed for CD45+ and CD3+ cell markers. Results. During heavy physical exertion, the proportion of CD45+ and CD3+ liver parenchyma cells increased by 3.2 and 2.9 times and perivascularly by 3 and 6.3 times (p=0.001) compared to the intact group. After the use of meldonium during heavy physical exertion, the percentage of CD45+ cells decreased in the liver parenchyma and perivascularly by 1.8 times (p=0.008), the proportion of CD3 cells decreased by 1.7 times in the liver parenchyma (p=0.001) and around the vessels by 2.3 times (p=0.004) when comparing data with intact animals. Histologically, inflammation and alterative changes in the liver tissue decreased. Conclusion. The use of meldonium during heavy physical exertion led to a decrease in the percentage of CD45+ and CD3+ cells due to a decrease in the degree of inflammatory infiltration of the liver tissue and the severity of the destructive process.