Isolation and characterization of vesicles from typical and genovariant strains of vibrio cholerae O1 El Tor

It was found that a typical strain and genovariants of Vibrio cholerae O1 release outer membrane vesicles into the environment at different growth stages when cultured in AKI medium. Preparations of vesicles were obtained by ammonium sulfate precipitation and visualized by electron microscopy. Using monoclonal antibodies and polyclonal antitoxic conjugate, the presence of lipopolysaccharide and outer membrane proteins OmpU and OmpT, as well as cholera toxin in these structures was determined. On the model of cell culture of two cell lines CHO-K1 and HuTu 80 it was shown that morphological changes of target cells characteristic for cholera toxin are observed in the presence of vesicles. The results obtained suggest that the content of outer membrane vesicles depends on the stage of vibrio growth, and the biologically active substances produced by them are capable of causing cell damage, thereby provoking an inflammatory reaction of cells in the host organism.