HPLC analysis of new biologically active compounds: azomethines, thienopyrimidines and dimethoxyquinazolinones

In this work, HPLC analysis of new biologically active compounds was carried out: azomethines, thienopyrimidines and dimethoxyquinazolinones. For these structures, such types of pharmacological action as anti-tyrosinase, antiviral and cerebroprotective have been established, which makes them promising compounds for pharmaceutical study. In order to establish the purity of the analyzed substances and develop methods for their chromatographic study, a separation analysis was carried out by the method of high-performance liquid chromatography with a reversed-phase variant. During the experiment, the optimal mobile phase was selected. The best options for analysis were achieved by using a mixture consisting of acetonitrile and 0.5% formic acid. Significant reliability of the results of primary pharmacological screening is ensured by the high purity of the substances studied, and this in turn allows us to develop laboratory regulations for obtaining a leader compound as an active pharmaceutical substance.