Influence of bone marrow transplant on the functional state of mast cells in lymph nodes

Purpose: to analyze the functional state of mast cells (MC) after bone marrow allografting in the early stages of the experiment. Material and Methods. The experiments were carried out on 40 outbred male mice, which were divided into 3 groups: 1st group — intact (n=10); 2nd — control animals, which were injected with 0.85 % sodium chloride solution (n=10); 3rd group was experienced (n=30), who received a cell suspension from another mouse under ether anesthesia into the tail vein. The state of mast cells was assessed by the method of staining with polychrome toluidine blue according to Unna. Results. 40 minutes after the introduction of bone marrow analog in the medulla, metachromatic mast cells are found near the vessels, their percentage (60 %) prevails over orthochromic (40 %). Mast cells are predominantly located in groups, especially near the vessels, in contact with each other. The morphometric parameters of mast cells change (diameter, 8.00–17.00, in intact ones — 6.00–12.00 μm). Two hours after the experiment, according to the degree of sulfation of heparin, mainly metachromatic mast cells are detected, their percentage is 92 % and actively degranulated mast cells (61 %), p<0.05, predominate. The size of mast cells changes (diameter — 3.00–9.00 μm; distance between cells — 29.00–53.00). One-way analysis of variance found that there is a significant effect of the experiment period on the change in the proportion of mast cells with metachromatic staining. Conclusion. The introduction of bone marrow transplant significantly changes the functional profile of mast cells.