Effect of beta-lactam antibiotics on microscopic parameters in the Allium-test

The work examines the effect of beta-lactam antibiotics (cefotaxime, ampicillin, augmentin) on the pathology of mitosis in the Allium-test. Research methods: Allium-test, cytogenetic analysis, statistical analysis. It was established that the use of individual tested beta-lactam antibiotics increases the percentage of pathological mitoses in the cell by 1.8-3.3 times compared with the value in the control. With the combined use of cefotaxime and Augmentin, synergism appeared, as a result, the value of mitosis pathology turned out to be at the level of the number in the control; minimally represented pathologies indicating damage to the mitotic apparatus. It was revealed that all three beta-lactam antibiotics tested had a pronounced statmokinetic effect. At the same time, with the joint use of cefotaxime and Augmentin, k-mitosis was not registered in dividing cells. Comparison of the spectrum of pathological mitoses in the variants of the experiment showed that the pathology ‘chromosome runaway/backlog’ in anaphase of mitosis dominates in all variants. An increase in the concentration of Augmentin and ampicillin caused the suppression of pathological processes in onion meristematic cells, a decrease in PM values was observed. An increase in Augmentin concentration does not affect the composition and spectrum of pathological mitoses; ampicillin has a decrease in the level of most of the recorded pathologies of mitosis.