Concerning the biologically active peptide components of hemolymph larvaes Galleria mellonella

The study of biologically active peptide components larval hemolymph of Galleria mellonella in condition of specific and nonspecific immunization them, ethanol extraction, isolation and the study by electrophoresis, and ion-exchange, HPLC and MALDI analysis, as well as determining the hemolytic and antibacterial activity. Alcoholic extracts of wax moth larvae do not have hemolytic activity on human erythrocytes and have a protective effect in conditions of oxidative stress in vitro. Investigation of antibacterial alcohol extracts showed the presence of bacteriostatic action against E. coli and Bacillus cereus. Conducted by MALDI analysis revealed the presence of the peptide compounds with a molecular weight from 875 to 18000 D. Among them were identified as the previously detections and unknown peptides. Some of them are found in specific immunization larvae acetone-1,1-dimethylhydrazone. Peptide components of hemolymph of larvae Galleria mellonella not only inhibit the growth of E. coli, but also have an impact on the rate of metabolic processes, resulting in the change of the enzymatic activity of cells of E. coli.