The influence of cytokines on karyological stability of cell cultures

Cell cultures in collections must preserve their original properties and genotypical characteristics, indicating their purity. The aim of this work was to study the properties of cell lines culture after long-term cryopreservation. The cells retained their original morphological features, the modal class of chromosome plates meets the passport requirements, and microplasma microbial contamination was absent. Growth, cytomorphological properties and caryological characteristics of the MDBK, ВНК-21-13/02 cell lines during at least 35 continuous cycles of stationary cultivation are stable. During the long-term storage in liquid nitrogen - 196 С the cell culture retains its biological properties after defrosting, if the cultivation corresponds to the requirements and can be used for production of vaccines to prevent animal viral diseases.