Effect of dalargin on the activity of Na, K-ATPhase in cerebral cortex synaptosomal membranes of rats in ischemia and reperfusion

Effect of a peptide dalargin on the activity Na, K ATPase in rats cerebral cortex synaptosomal membrane during ischemia and reperfusion was studied. Ischemia caused by occlusion of the two carotid arteries, leads to inhibition of enzyme activity. The degree of decrease in activity of Na, K ATPase dependes on the duration of ischemia (30, 60, 90 min). During reperfusion (60 and 90 min), after 60 min of ischemia, the activity Na, K ATPase increases relatively ischemic level. Dalargin intraperitoneal injection (30 min) before ischemia with dose 0,5 mg/kg, but not with dose of 0,1 mg/kg, prevents the inhibition of the enzyme during ischemia.