Influence of doxorubicin on adhesive properties of E. coli

Influence of antineoplastic drug doxorubicin and amikacin, the aminoglycoside family on adhesive activity of Escherichia coli was studied. Antimicrobial activity (minimum inhibitory concentration-MIC) of both drugs against experimental strains using serial two-fold dilution method was determined. Susceptibility of E.coli to amikacin in the presence of Ѕ and ј MIC doxorubicin was studied. After 10 passages in beef-extract broth with constant and increasing doxorubicin concentrations in the presence of Ѕ and ј MIC doxorubicin, the adhesive activity of initial and passage variants according to their ability to absorb human erythrocytes I(0) Rh+ was determined. It was observed that experimental strains were susceptible to amikacin (MIC 1,5-6,2 mkg/ml) but were resistant to doxorubicin (MIC 1000 mkg/ml). Subinhibitory concentrations of this cytostatic (Ѕ and ј MIC) raised the sensitivity of experimental strains to amikacin and differently effected on adhesive activity of passage variants of E.coli.