Effect of pulsed magnetic fields on the expression levels of tumor suppressor genes in human T98G glyoblastoma cell line

Aim: to study the effect of a pulsed magnetic field (PMF) on the expression of key tumor suppressor genes, such as APC, MLH, and MGMT in human T98G glioblastoma cell line. Material and Methods. The PMF with the intensity of 15 and 300 mT was used alone and in combination with ionizing radiation at a single dose of 10 Gy. To perform ionizing radiation, Theratron Equinox 60Co unit Best Theratronics Ltd., Ottawa, Canada) was used. The source of the pulsed magnetic field was Neuro-MS / D Therapeutic Advanced device of the Neurosoft company. Live and dead cells were determined in NanoEnTekJuliFl cell counter (Korea) using a 0.4 % trypan blue solution to stain dead cells. Total RNA was extracted according to the protocol of the manufacturer Trizol with changes: the aqueous phase was separated with Trizol reagent twice. The quantitative measurement of the isolated RNA was carried out on a Qubit 2.0 fluorimeter using a kit of reagents with the Quant-iT RNA AssayKit RNA intercalating dye (Life Technologies, USA). The expression of MLH, APC, and MGMT genes was evaluated by RT-PCR using a CFX96 amplifier (BioRad, USA). Data were analyzed using the cycle threshold (Ct) method with normalization for TBP gene expression in each sample. Relative expression of the genetic locus (Exp) was calculated by the 2-ACt method. Statistical analysis of the results was carried out using the Statictica v10 software package. Results. One day after exposure to PMF, significant differences in the MGMT expression level compared to the control were found (p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }