Impact of nitrogen sources in the nutrient medium on the growth and cellulase activity of streptomycetes

Автор: Shirokikh I.G., Bokov N.A., Nazarova Ya.I.

Журнал: Известия Коми научного центра УрО РАН @izvestia-komisc

Статья в выпуске: 5 (51), 2021 года.

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Biomass accumulation and cellulase activity were determined in two promising soil isolates during liquid phase cultivation depending on organic (peptone, casein hydrolysate) and mineral (NH4Cl, NaNO3, NH4NO3, (NH4)2SO4, KNO3) nitrogen sources. The only source of carbon in the medium was straw (1ob.%). Using two-factor analysis of variance, it was found that the production of cellulase complex enzymes is equally influenced by the nitrogen source (F= 225,92; p4NO3 provided enzymatic activity in Streptomyces sp. Mb 4-2 is 912,82±60,40 units/10 min /g, and NH4Cl in Streptomyces sp. 1.3 NH4Cl is 531,37±27,25 units/10 min /g, which is 58-481 % and 34,5-409% more, respectively, than on other tested nitrogen sources. The accumulation of biomass during growth was more dependent on the nitrogen source (F= 65,47; p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }

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Streptomyces, nitrogen sources, cellulose degradation, cellulase activity, plant materials and waste

Короткий адрес: https://sciup.org/149139326

IDR: 149139326   |   DOI: 10.19110/1994-5655-2021-5-61-65

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