Influence exopolysaccharides of lactic acid bacteria on some hematological parameters rats in modeling burn

We studied the effect of exopolysaccharides (EPS) of lactic acid bacteria Lactococcus lactis B-1662 and Streptococcus thermophilus on various forms of leukocytes - leukogram (eosinophils, neutrophils, lymphocytes, monocytes) in female rats during the healing process of burn wounds. For the experiment, the rats were divided into several groups: group 1 - animals without a burn, group 2 - animals that caused a burn, group 3 - animals that caused a burn, followed by applying a commercial preparation of 5% dexpanthenol, group 4 - animals, after a burn, a 0.6% EPS solution of L. lactis B-1662 was applied, group 5 - animals to which a 0.6% solution of S. thermophilus EPS was applied after the burn. A degree IIIa burn was simulated under ether anesthesia in the interscapular space of the rat with the bottom of a boiling water tube for 30 seconds. The application of bacterial EPS was performed immediately after the burn was applied and then daily for 28 days of the experiment. The results of leukogram showed that EPS of Lactococcus lactis B-1662 and Streptococcus thermophilus affect blood formation, stimulating the acceleration of the completion of the infectious process. The studied EPSs had the most pronounced effect on eosinophils, monocytes, segmented neutrophils and lymphocytes compared with a group of animals without treatment. In groups of rats with treatment with bacterial EPS, the percentage of leukocytes recovered to the physiological norm earlier than in the group without treatment. Changes in the morphology of the blood leukocytes of animals were not established in any of the groups during the experiment. The ability of these EPSs to stimulate the maturation of some forms of leukocytes during the healing of burn wounds in animals, and also, as it was established earlier, that EPS data contributed to a more rapid healing of wounds compared to a commercial drug, it can be used in medicine and veterinary practice in the future.