Effect of electrical stimulation on the content of pAkt in myocyte cell culture under hyperglycemia

Introduction. The problems of studying the molecular mechanisms of pathogenesis, insulin resistance and fi nding new ways to correct them are still relevant. Aim: To study the eff ect of electrical stimulation on the amount of pAkt in a cell culture of C2C12 mouse myoblasts cultivated under hyperglycemic conditions. Material and Methods. We studied the eff ect of electrical stimulation on the amount of pAkt in a cell culture of C2C12 mouse myoblasts cultivated under hyperglycemic conditions. Cells were cultured in a medium containing 25 mM glucose and subjected to electrical impulse stimulation for 2, 6, and 24 hours. Results. It has been shown that the cultivation of C2C12 myoblasts in a medium with excess glucose is accompanied by a decrease in the amount of pAkt in cells, while pulsed electrical stimulation from 2 to 6 hours increases the concentration of this enzyme and restores the sensitivity of its phosphorylation pathways to insulin. Conclusions. The results obtained suggest that the contractile activity of muscle cells contributes to the restoration of cell sensitivity to insulin and the ability to absorb glucose, using the same regulatory and transport pathways that are affected i the development of type 2 diabetes.