Influence of the dihydroquercetin and arabinogalactan composition on the contraction of rat'S portal vein smooth muscle cells

The aim of the study was to evaluate influence of the dihydroquercetin (DQ) and arabinogalactan (AG) composition on the contraction of murine portal vein smooth muscle cells (SMC). The contractile activity of the rat portal vein segments was investigated by the mechanographic method by using a device with mechano-electrical transducer (FT10g, World Precision Instruments, Inc. USA). The SMC contractile responses to 40-mM KCl and 10-мМ phenylephrine were evaluated after 20 min pre-incubation with DQ (0.001%), AG (0.005%), and their composition with ratio of 1:5 (0.006%). The amplitude of KCl-induced contraction after pre-incubation with DQ was 61.0+5.2% of the initial contraction. Treatment with the composition of DQ and AG reduced the inhibitory effect of DQ on the KCl-induced contraction. Studied substances and their composition suppressed SMC contractions induced by phenylephrine.