Influence of light stress on the metabolic effects of runing loads in mice with a model of diabetes mellitus type II

Rationale. The influence of the light factor on the metabolic effects of running loads in mice with type II diabetes mellitus, used in the dark phase of active life, was studied. Methodology. To form a model of the disease, a high-fat diet was used, physical activity in the form of forced running was carried out for 4 weeks. The content of glucose, insulin and cortisol in plasma was determined biochemically, GLUT-4 in m.gastrocnemius muscle tissue was determined by Western blotting Results. Forced physical activity in the form of daily treadmilling has a number of pronounced effects on metabolism in mice with type II diabetes mellitus. First of all, this is manifested in a decrease in body weight. Also, physical activity is accompanied by an increase in the rate of glucose uptake and an increase in insulin concentration. All of the above indicates the normalization of carbohydrate metabolism under the influence of regular physical activity and the involution of changes characteristic of type II diabetes. Moreover, the mechanism of these changes is associated with one of the main pathogenetic factors of diabetes - a fat diet in mice is accompanied by a decrease in the content of GLUT-4 in muscle tissue, and forced physical activity, on the contrary, by its increase. Lighting enhanced the metabolic effects of forced physical activity. Conclusions. Physical activity, implemented taking into account circadian rhythms, is a promising way to influence metabolic processes, which is important for finding new ways to correct metabolic disorders. However, the influence of factors combining circadian rhythms and illumination must be taken into account both when designing experiments and when using physical activity in the rehabilitation of patients with type II diabetes mellitus.