Influence of methylene blue on human spermatosoid mobility in vitro

Introduction. Contemporary demographic situation in Russian Federation is characterized by low birth rates. According to data provided by V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology the percentage of infertile couples in our country is over 17%. Reproductive dysfunction therefore becomes medically and socially important. However, treatment of this dysfunction hasinadequate efficacy. Search for medicationswhich may help conserve fertility is a relevant problem of andrology and reproduction.e goal of thisstudywasto evaluate the effect of methylene blue (MB) preparation on sperm motility and viability. Materials and methods. e study was performed on ejaculated human sperm obtained by usual means. Motility and viability parameters were evaluated according to 5th edition of WHO guidelines. One milliliter was taken from every semen sample for experiment and control. MB was introduced to experimental sample tube until 1 nM concentration was obtained...