The influence of monoiodacetate on the thermotolerance of Clavibacter michiganensis ssp. sepedonicus and Sacharomyces cerevisiae

Abstract - To search the antiseptic agents capable to decontaminate the plants from pathogens the combined effect of moderate heat shock (45°С) and glycolisis inhibitor monoiodoacetate (MIA) on survival of potato pathogen Clavibacter michiganensis ssp. sepedonicus (Cms) and yeast Saccharomyces cerevisiae was studied. Under optimal temperature cultivation (26°С) MIA had no toxic effect on S. cerevisiae but decreased viability of Cms. The lethal effect of MIA significantly increased during heat treatment at 45°С. MIA in the range from 0.1 to 1 mM decreased the thermotolerance of Cms and S. cerevisiae cells in 10-10000 folds in dependence from time of treatment. A minimal concentration of MIA capable to affect the thermotolerance was 0.1 and 0.3 mM for S. cerevisiae and Cms, respectively. The effect of MIA on Cms and yeast survival during heat shock was stronger in logarithmic phase than in stationary ones.