Organic fertilizers and natural zeolite influence on the Brassica napus pollen morphometric characteristics

The purpose of research is a comparative study of the morphometric characteristics of Brassica napus pollen grown on variants with different levels of nutrition, and the identification of features of the formation of spring rapeseed productivity. The influence of organic fertilizers and natural zeolite on the morphobiological characteristics and productivity of spring rapeseed was revealed. The work was carried out during 2018-2020 on the experimental field of Yelets State University named after Bunin. Morphological characteristics of Brassica napus pollen (polar axis, equatorial diameter, shape index, exine perforation pattern, and perimeter of perforation zones) were also proposed, which were studied using JEOL JSM-6390 scanning electron microscopy. It was shown that the pollen grains of Brassica napus had an oblong-ellipsoid shape. Pollen grains were round with straight sides in polar view, elliptical in equatorial view. For the equatorial axis among fertilizer applications, the coefficient of variation ranged from 8.47 to 12.01 % compared to the control (18.35 %). For the outer perforation perimeter of pollen grains, the minimum values ranged from 1.68 to 1.95 µm, while the maximum values ranged from 4.34 to 7.12 µm compared to the control (1.34; 5.68 µm). The pollen grain shape index of Brassica napus varied from 1.96 to 2.07 compared to the control (2.03). In general, when using organic fertilizer and zeolite, the morphometric parameters of pollen grains were significantly improved.