The effect of different fractions of essential oil of Pleurospermum uralense Hoffm filopodia on the growth of bacteria

In the study the results of research of compo-nent structure of essential oil of seeds of Pleu-rospermum uralense Hoffm, the family plant Um-brella, growing in Siberian region (Krasnoyarsk re-gion) are for the first time presented. The plant was rather widely applied in traditional medicine as a helminthicide, at pulmonary diseases including pulmonary tuberculosis. The essential oil was re-ceived by the method of an exhaustive water vapor distillation from air and dry raw materials. In the course of distillation oil was fractioned depending on the time of its allocation. The results of chroma-tography-mass-spectrometry analysis showed that the studied samples of essential oil contain more than 30 components, 34 of which, making 98.4-99.8 % of the sum of all components of oil depend-ing on a sample, were identified by us. The main components were α-pinene, β-рhellandrene, Z-azaronе, E-azaronе. It was noticed that with aug-mentation of duration of process of release of oil concentration mono- and sesquiterpenes decreas-es, and the concentration of oxygen-containing components of oil among which all three isomers of azaron occurred: α-azaron ((E)-azaron), β-azaron ((Z)-azaron) and γ-azaron increased. It was known to be the change of structure influences the proper-ties of essential oil. The results of research of anti-microbial activity showed that the essential oil of seeds of P.uralense had weak antibacterial proper-ties. And essential oil, undivided on fraction (inte-gral), and also its 1-st fraction only Staphylococcus aureus contained growth. The second fraction of oil containing the greatest in comparison with other fractions, amount of alpha pinene, had bacteriostat-ic effect in relation to Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The component structure of the 3-rd fraction pro-moted control of body height of all bacteria taken in an experiment. The 4th and 7th fractions were indif-ferent to bacteria. The fifth fraction of oil contained the growth only of Pseudomonas aeruginosa, the 6th fraction did not operate on Pseudomonas aeru-ginosa and Escherichia coli, but had bacteriostatic effect on other bacteria. Thus, results of the con-ducted researches showed that allocating separate fractions of essential oil in the course of its receiv-ing, it was possible to receive the models of differ-ent referred action in relation to this or that strain of bacteria.