Influence of growth regulators in clonal micropropagation of the blackberry

Data on the effect of growth regulators in the clonal micropropagation of blackberry. The study of the organo-genesis of the blackberry plants of the Adrienne variety at the stage of «micropropagation proper» on theMurashige-Skoga nutrient medium (MS) using cytokinins 6-BAP (0.5 mg/l and 1.0 mg/l), cytodef (0.1 mg /l and 0.2mg /l) and adaptogen epin (0.1 mg/l).The positive effect of epin on the biometric characteristics of the regenerative plants of the blackberry varietyAdrienne. The maximum number of shoots (an average of 8.1 pcs.) formed using a cytodef at a concentration of 0.2mg/l. The greatest number of shoots (8.6 pcs.) was observed when cytodef was added to the nutrient medium at aconcentration of 0.2 mg/l and epin (without epin – 7.5 pcs.).The total length of shoots largely depends on the concentration of cytokinins. The maximum total increase isobserved when the cytodef is used at a concentration of 0.2 mg/l and reaches an average of 6.6 cm. Adding 0.1mg/l of epine to the nutrient medium contributes to an increase in the total length of the shoots. More active stim-ulation of shoot formation in the blackberry variety Adrien with the use of cytokine cytodef even in significantlylower concentrations in comparison with 6-BAP.