Influence of nutrient medium composition on regeneration of some Clematis L. cultivars in vitro

The aim of the work was to optimize cultivation techniques at the stage of actual micropropagation of Clematis L varieties. N.V. The studies were carried out in 2021-2022 in the laboratory of plant biotechnology of N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences (GBS RAS). Varieties of various garden groups of clematis were chosen as objects: Princess Diana, Madame Julia Correvon, Yubilejnyj 70, Multi Blue. In the course of the study, the influence of genetic characteristics and the composition of the nutrient medium on the regeneration potential of Clematis L. was shown. It was established that on the nutrient medium DKW (Driver and Kuniyuki, 1984) with the addition of 0.1 mg/l BA (6-benzylaminopurine), Clematis L. regenerants were characterized by active growth of microshoots and the highest height. It was 5.0 ± 0.2 cm for Princess Diana, 6.3 ± 0.1 cm for Madame Julia Correvon, 2.1 ± 0.1 cm for Yubilejnyj 70, and 2.8 ± 0.1 cm for Multi Blue. The maximum multiplication factor was noted for the Princess Diana variety (3.7 ± 0.1), the minimum for the Multi Blue variety (2.4 ± 0.1). When studying the effect of growth regulators and their concentrations during cultivation on DKW medium, it was shown that 2-iP (2-isopentyladenine) at a concentration of 1.5 and 2.0 mg/l was more effective than BA at the same concentrations. At the same time, the highest height (8.6 ± 0.2 cm) and multiplication factor (6.7 ± 0.2) were observed in the Madame Julia Correvon variety on a nutrient medium with the addition of 1.5 mg/l BA. The multiplication factor of variety Yubilejnyj 70 reached its maximum at 2.0 mg/l BA (4.2 ± 0.1), variety Multi Blue - at 1.5 and 2.0 mg/l 2-iP (4.0 ± 0 .1 and 4.3 ± 0.1, respectively).