The effects of stress-factors of different nature on gene expression stress response in male Drosophila melanogaster

In male Drosophila melanogaster the expression of reporter gene of green fluorescent protein GFP was analyzed. This protein is under the transcriptional control of promoters of common response to stress genes ( D-GADD45, Stat92E- D ), genes of innate immunity ( Defensin, Drosomycin, Metchnikowin ), gene of detoxification of xenobiotics ( GstD1 ), genes of heat shock response ( Hsp22 and Hsp70 ). Analysis was performed after the exposure to stressors of different modalities (bacterial infection, fungal infection, hypergravity, starvation, dehydration, paraquat, dioxin, toluene, formaldehyde, hyperthermia, cold stress) at different doses and at different times after exposure. Analysis of the expression level of GFP-reporter allowed to determine the spectrum of factors that are capable of activating certain genes of cellular stress response in a model of Drosophila melanogaster in vivo. The obtained experimental model can be used to solve problems of bioindication of toxicants in laboratory conditions.