Led lighting effect on the adaptation of water mint regenerant plants in an environmental chamber

Growth rate, plant biomass and concentration of beneficial compounds largely depend on the quality and intensity of lighting. The purpose of research is to study LED lighting on the process of adaptation of regenerated watermint plants using a digital climate camera. During research, the object studied was microclones of watermint (Mentha aquatica L.). Watermint microclones were grown by in vitro cell and tissue culture in the laboratory of the Russian State Agrarian University - Moscow Timiryazev Agricultural Academy. Subsequently, a set of measures was carried out to adapt plants in vitro to ex vitro conditions using a digital climate chamber produced by VIM (Russia). Adaptation of regenerated plants was carried out using two options of light-emitting diode lighting (LED): B:G:R ~ 16 : 42 : 39 and B : G : R ~ 26 : 66 : 49 for 24 days. Assessing the intensity of plant growth over time by the 24th day, significant differences were revealed between the LED lighting options. When studying the quantitative content of photosynthetic pigments, it was found that different light intensities did not affect the accumulation of total chlorophyll and carotenoids in watermint leaves. A significant influence of the intensity of illumination from LED light sources on the root formation of watermint has been established. As a result of assessing the effectiveness of using different spectra of LED lighting to increase the rooting of watermint under ex vitro conditions, a technological method of using LED lighting with a spectral composition B : G : R ~ 16:42:39 and a total PAR of 100 mmol/m2s was determined.