Influence of triton X-100 on the PCR-profiles of enzyme genes and isozyme patterns in Beta vulgaris L. during germination

The purpose of this work is to study the effect of the detergent Triton X-100 on the PCR-profiles of enzyme genes in germinating beet seeds (Beta vulgaris L.). In this study, we used hybrid seeds obtained by crossing sugar and red table beets. PCR-profiles of enzyme genes in the control and experimental (treated with 0.1% Triton X-100 solution) seed groups were compared from the 18th hour to the 114th hour of germination with an interval of 24 hours. In addition, in ungerminated seeds, both the PCR-profiles of enzyme genes and the isoenzyme spectra determined by these genes were analyzed too. TX-100 influences ontogenetic changes in the Gpi and Me PCR-profiles differently. It can be assumed that TX-100 induces changes in the membrane-associated structures regulated processes. The dual effect of detergent TX-100 on the enzyme genes PCR-profiles and gene expression indicates that TX-100 makes its primary impact on the genome.