The influence of carbohydrates on the biosynthesis of nucleic acidsand proteins in germinating wheat grains of the variety 'Buryatskaya ostistaya’

The germination medium influences on germination rates of wheat grains of Buryatskaya ostistaya” variety was studied. The seeds were germinating in solutions of carbohydrates: simple (glucose, sucrose) and comlex (arabinogalactan). The temperature of germinating made 24-26 °C. The duration of germination was 3 days. The concentration of sugars in the solutions for germination was 0.1 % (masses). The total content of nucleinic acids in sprouted grains of wheat and the general content of proteins were determined by spectrofotometric method. It was revealed that the environment for germination significantly influenced the indicators of germination of seeds of wheat of the variety Buryatskaya ostistaya. Biosynthesis of nucleinic acids amplified at germination of seeds in solutions of all carbohydrates in comparison with control (water). When the grains germinated in the solutions of simple carbohydrates (glucose, sucrose), nucleic acid content was almost the same, and the complex carbohydrate solutions (arabinogalactan) provided sharp increase in the content of nucleic acids in the acrospires. In general for the sprouted wheat grains beneficial effect of the environment for germination on the total content of nucleic acids enhanced in the series: water - glucose - sucrose - arabinogalactan. The highest protein content in the acrospires was found for the sucrose solutions of the studied sugars, and the lowest was for the arabinogalactan solutions. The sequence of increase in protein content in germinated wheat grains depending on the medium for germination was: water - arabinogalactan - glucose - sucrose. Compared to dry grains, in the acrospires germinated in the glucose and sucrose solutions the rates of increase in the content of nucleic acids and proteins almost coincided. The greatest difference between these processes was observed for the solutions of arabinogalactan: the content of nucleic acids increasedsharply in comparison to the other solutions, and the protein content, in contrast, decreased in comparison to the solutions of simple carbohydrates. During the germination of grains in sugar solutions, the content of nucleic acids increases compared to dry grains, and protein decreased.