Hemp cake influence on digestibility and rumen metabolism in vitro

The purpose of research is to study the effect of various doses of hempseed cake on the in vitro digestibility of the dry matter of concentrated feed and rumen metabolism. The experiment was carried out on the basis of the Center for Collective Use of the Federal Scientific Center of the BST RAS on the "artificial scar" model using the ANKOM Daisy II incubator (modifications D200 and D200I). 4 samples were prepared in three repetitions: Control group - grain mixture 100 %; Group I - grain mixture 86 % + hemp cake 14 %; Group II - grain mixture 80 % + hemp cake 20 %; Group III - grain mixture 74 % + hemp cake 26 %. It was found that the introduction of hemp cake in different dosages into a sample with dry matter of barley grain contributed to its digestibility by 1.5 %, 3.81 and 4.82 % compared to the control. At the same time, the sample in group III, containing 26 % hemp cake, was distinguished by the best digestibility. The content of total nitrogen in the rumen fluid of group III was 36.4 mg/%, 37.8 and 35.0 mg/% more than in the control, I and II groups, respectively. The control sample was inferior to I and III groups by 8.28 and 17.14 %. The largest amount of ammonia nitrogen was noted in the rumen fluid from group III, by 0.7 mg/% relative to the control. content by 41.71 %. As for the protozoa, there is a consistent increase in their number in all experimental groups - by 24.24 %, 52.27 and 139.39 %, in comparison with the control.