Reproductive quality of roosters with normal and modified genome

To date, progress has been made in the production of transgenic poultry: effective gene constructs have been obtained, and efficient systems for recombinant DNA delivery into target cells have been created. However, when breeding a genetically modified poultry, problems may arise with transgenic offspring, in particular its low viability or limited number. This paper is the first to report on histological structure of the testes and the composition of spermatogenic cells in the seminiferous tubules of transgenic roostersand on the quality and fertilizing ability of their semen. The study was carried out on transgenic roosters which were obtained by us in different ways based on chicken ( Gallus gallus L.) Pervomaiskaya breed and their non-transgenic analogues (vivarium of Ernst Federal Science Center for Animal Husbandry, 2017-2018). Group I was transgenic roosters after introduction of the lentiviral vector pWRSV into chicken embryos in vivo ( n = 4); Group II was transgenic roosters obtained by transplantation of transformed in vitro donor spermatogonia into the testes of sterile recipient roosters ( n = 5)...