The choice of optimal conditions of enzymatic hydrolysis of collagen-containing raw materials

The research objective was to pick up the parameters of enzymatic hydrolysis of collagen-containing raw materials for the achievement of high values of the extent of hydrolysis with subsequent allocation of glycosaminoglycan from it. The re-search problems were to pick up enzyme preparations for hydrolysis of raw materials, to define enzyme-substrate ratio of enzymes, duration, temperature and pH process. Raw material for allocation of glycosaminoglycan was collagen-containing raw material - pork cartilage. For carrying out en-zymatic hydrolysis commercial enzyme preparations the tryp-sin and chymotrypsin (JSC “Samson-Med”) received from KRS pancreas were used; the papainase received from mel-on tree of Carica papapya (AppliChem); KK collagenase re-ceived from trade species of crabs (The Pacific Institute of Bioorganic Chemistry) which in different degree split collagen, and also its communications with carbohydrates. For the as-sessment of potential opportunities of used raw materials the analysis of its chemical composition was carried out. For allo-cation of glycosaminoglycan from raw materials enzymatic hydrolysis was used. For fuller impact of enzymes on raw materials and increases of glycosaminoglycan exit crushed and cleared raw materials were washed out by 0.1 M phos-phatic buffer at the temperature of 60-65 ° C within 30 minutes. As criterion of enzyme preparations efficiency the extent of hydrolysis of protein was used which was estimated on the dynamics of amine nitrogen accumulation. Hydrolysis was carried out in optimum conditions for each enzyme prep-aration. By the results of conducted researches enzymes (papain and collagenase) for enzymatic hydrolysis of collagen containing raw materials rich in glycosaminoglycan were picked up, and process parameters providing high rate of extent of hydrolysis of raw materials was determined. Con-secutive introduction of chosen enzymes at pH of 6.5-7.5 at the temperature of 40 ° C and conducting hydrolysis within 6 hours allowed reaching the extent of hydrolysis of raw materi-als of 77-78 %.