The allocation and characterization of poplar bacterial endopytes

The aim of the work was to isolate endophytic bacte-ria from the wood of the laurel poplar (Populus laurifolia Ldb.) and fragrant poplar (Populus suaveolens Fisch. The objectives of the study were to identify endophytic bacteria from the wood of the laurel poplar; isolate en-dophytic bacteria from the wood of the fragrant poplar; to identify microorganisms. Wood samples were taken in early October with sterile increment borer. The wood sawdust from the central part of the trunk was investi-gated by the method of growth. Meat and peptone agar was used as a nutrient medium. The sawdust of the laurel poplar is overgrown with bacteria more intensive- ly, in comparison with the poplar fragrant. To determine enzymatic (biochemical) activity of studied bacteria, differential-diagnostic media of Guiss were used. For isolates of bacteria the identification was carried out by sequencing fragments of the 16S рRNA gene. Bacterial DNA was isolated from the pure cultures of the bacterial strains...