Isolation and identification of epizootic diarrhea virus in pigs under outbreak at a large farm

Epizootic diarrhea fever (EDF), characterized by acute enteritis and hard intestinal disorders, is caused by the virus of the same group of С oronavirus ( Coronaviridae ) as the virus of transmissible gastroenteritis of pigs, coronavirus of cats and dogs, and human 229E strain. From 2005, at the territory of the Russian Federation the outbreaks of the disease were recorded, clinically similar to pig transmissible gastroenteritis. The infections caused a loss of piglets, in some cases up to 80 %. However, the sows in such farms have been vaccinated against the transmissible gastroenteritis of pigs. The aim of our study was to isolate in cell culture and identify an infectious agent that causes the outbreaks with diarrheal syndrome in pigs. From the pathological material of 3-5-day-old piglets with the pathology a RNA virus was isolated and identified as the virus of epizootic diseases of pigs. Virus infectivity in the fifth passage in Vero cell culture was 10 3,50 ТCID 50/sm 3. The isolate was named as BS-08. To date, there are 25 successive passages of the virus in cell culture. A citopatic effect is registered in 24-48 hours, and the infectious titer (titer of citopatic effect — TCE) makes 10 4,75-10 5,50 TCID 50/sm 3.