Interaction of big celandine extract and plasmid DNA, excreted from the clinical strain Escherichia coli

Search for compounds which possess antibacterial activity and have an effect on plasmid DNA, is one more of the important problems of applied medicine. Natural substances are the most desirable candidates for this role. Plasmid DNA excretion was carried out from the strain Echerihiacoli, derived from patient N., who suffered from chronic pyelonephritis, in the bacteriological laboratory of the Regional clinical hospital of Saratov according to the modified method Birnboim and Doli. The calculation of alkaloid concentration was made, e.g. Sanguinarin and Cheleretrin contained in celandine extract. The devised method has been tested on five celandine tincture samples, stored for the research of the stability of the content of alkaloids and other quality factors of the given preparation. In so doing the content of the alkaloid sum in tincture changes within the limits of 1 %. Abatement of plasmid DNA concentration after the cell treatment by celandine extract is revealed.