Drosophila body mass on ad libitum medium correlates with lifespan increments observed as the phenotypes of circadian rhythm genes overexpression in the fat body

The rhythmic functioning of the molecular circadian clock in various tissues and nutrition has a significant impact on the duration and quality of life, as well as the rate of aging of organisms. It is known that body weight correlates with the expression of various genes and the risk of death at different ages. In this study, an attempt was made to identify patterns in realization of genetic information under the influence of various factors, in particular, the ability of ectopic expression of circadian rhythm genes in the fat body and muscles to influence body weight in conditions when media contain different concentrations of protein. We used transgenic Drosophila melanogaster lines carrying UAS sequences in promoters of circadian rhythm genes ( cry , per , tim , clk , cyc ), that enhance expression, and lines that allow to induce the expression of tissue-specific GSG-311-2 (muscles) and P {Switch1} 106 (fat body). Drosophila body mass was measured on “Mettler Toledo” analytical scales, and a correlation with lifespan increments in response to overexpression of circadian rhythm genes from previously published data was established. A strong correlation was found between the increments of body weight and lifespan in the case of overexpression of circadian rhythm genes in the fat body against the background of a protein-rich diet; according to Spearman, the correlation coefficient was 0.955. Based on the results obtained and data on the high evolutionary conservatism of the signaling pathways involved in the process (human signaling pathways are evolutionarily close to Drosophila cascades), generalizations were made regarding the negative consequences of using high-protein diets to reduce the body weight against the background of circadian rhythm disorders that are widespread in megacities.