Way of stimulation reproductive functions of minks

T h e p r o b lem of imm u n e r e a ctiv ity reg ul atio n h a s gre a t im p or ta n ce, b o th i n th e o r e tica l, an d in p r a ctica l a sp e cts. T h e se a r ch of n e w im m u n o a ctiv e p r e p a r a tio n s is o n e o f a ctu a l tas k o f z o ot ech n ica l a n d v e t e r in a r y m e d icin e . T h e i m p o rta n t p la ce in r egu la tio n of the immune answer is stimulation of immunity [1]. In sp ite of wid e p r a ctica l u se o f v a rio u s stim u la to r s in the a n im a l ca t tle b r e e d i n g , m a n y qu e stio n s of th e ir r atio n a l a p p lica tio n a r e in suff icie n tly studied. Such questions as the most optimum dosages, f r equ e ncy r a te s of a n inj e ctio n a nd inte rv a ls a r e betw e e n th e m . In th is r ega r d, th e t a sk se t b y u s in th is w o rk of s tu d y in g of stim u lat in g inf lu en ce of a b io stim u la tor n uk le o p e pt id , a llo w s to f in d o ut a n um b e r of qu e stio n s of influence of these substances o n a n o r ga n is m a n d to d e f in e r atio n a l m e th o d s of th e ir u s e in fur farming [2].

Tests of preparation's action were carried out in February-M a r ch , 2 0 13 on a m ink of bree d S k a n b la c k in o p e n j o in t sto c k m ark e t e ntity " O kto b e r fu r fa rm ". In Feb r u a r y 4 gr o u ps of fem a le s u p to 4 0 h e a d s b y th e p r in cip le o f a n a l o gs a n d 2 g r o u p s of m ale s u p to 30 h e a d s we r e f o rm e d in Fe b r u a r y . P r e p a r atio n in a d iff e r en t d o sa ge w a s se t in 2 we e k s pr io r to r utting by a three-day course and further 1 time in 7 days, including during rutt ing . A n im als of co ntr o l grou p d id n 't r e ce iv e th e p r e p a r atio n . T h e sch eme of f o rm atio n of f em a les a n d m a le s gr o u p s of minks is shown in figure 1.

Figure 1 - Scheme of experience

After preparations’ introduction to adult males it was considered: the quantity a cage in day, quantity of the females’ leaper, % littered females from number leapered, % being pro- empty and unsuccessful given rise females, quantity of puppies, morphological and biochemical indicators of blood. The quantity of puppies to a jigging, quantity of dead born puppies was considered at females.

RESULTS OF RESEARCH

Results of an assessment of males on sexual activity are presented in table 1. As we can see in the table 1, during rutting by males of skilled group it was made about 10,8 coitus that is more than control result for 4,6%. Respectively, the quantity of females leapered with one male in skilled group, also exceeded the control's result for 6,6%.

After females' whelping, impregnating ability of males' sperm was studied. It is seen from table 2, on one male of skilled group littered down 4,2 females that is 11,9% more, than in control.

Table 1 - Sexual activity of minks' males

Indicator	Group
	Control n=30 animals.	Skilled n=30 animals.
Number of coitus , time	10,3±0,39	10,8±0,47
Number of leapered females	5,6±0,19	6,0±0,34

As a whole efficiency of males use is defined by number of the puppies received from each of them. This indicator, in its turn, depends on a great number of others: from number of the leapered females, fertility, % of pro-emptied and unsuccessful whelping.

Table 2 - Impregnating ability of sperm of males of minks' males

Indicator	Group
	control	skilled
Littered down females on 1 male	3,7±0,32	4,2±0,26
Number of whelping , %	61,9±3,63	74,3±3,80*
Number of pro-emptied, %	35,2±4,11	25,7±3,81
Number of females,%	21,3	18,7

*- Р<0,05.

The reliable difference between groups on percent of whelping is observed, in favor of skilled for 16,6%. The greatest specific weight of being pro-empty females - 35,2% was noted in control group that is 27% more than result of skilled group. The similar tendency is observed and on an indicator of females of NBR, such females in control group were registered 21,3% that exceeds result of skilled group for 13,9%. The analysis of the females’ fertility leapered with stimulated males is presented in table 3.

Table 3 - Females' fertility leapered with males stimulated by the preparation

Indicator	Group
	control	skilled
Received puppies on a male, all animals	25,0±1,69	25,9±2,43
on leapered female	4,2±0,29	4,4±0,24
on whelped female	6,2±0,39	6,8±0,31*
received puppies in total, animals	750	777

* - P<0,05.

Table 4 - Results of females' whelping

Indicator	Group
	control	The 1st skilled	The 2nd skilled	The 3d skilled
Pro-emptied females,%	10,0	10	3,3	3,3
Females NBR, %	10	6,6	3,3	6,6
received puppies, animals on the leapered female	4,4±0,28	4,6±0,34	5,4±0,20**	4,9±0,24
on whelped female	5,3±0,31	5,7±0,18	6,2±0,29*	5,9±0,23
total puppies, animals	131	145	168	152

**-Р<0,005.

As it is seen in the table 3, the bigger quantity of puppies was received from males of skilled group - up to 25,9 animals on everyone on the average that is 4% higher, than in control. More puppies on littering female were received also in skilled group, at a reliable difference with control in 8,8%.

The analysis of the females' fertility presented in tab. 4, showed that the bigger quantity of being pro-empty females was noted in control group and 1st-skilled, receiving 2,5% nucleopeptid solution. At the same time in the 2st and 3rd skilled groups this indicator was 6,7% lower. The greatest number of unsuccessful born females is registered in control group – 10%, the small percent of not well-being was noted in the 2nd skilled group receiving of 3% solution of a preparation. The best result of the received puppies on the leaprerd female was registered in the same group, at a reliable difference (Р<0,005) with control in 18,5%, on a littering female for 14,5% (Р<0,05)

As a result the greatest number of live puppies was born in group of the females receiving 0,1 ml for kg of the live weight of 3% of water solution of a preparation-168 animals.

Economic efficiency of using of a nucleopeptid preparation was defined by increase of number of the received puppies from females of the main herd.

To set a prime cost of an issue at minks kit crop R.T.Safiullin, M.A.Musatova's [3] formula was used according to which the cost of a puppy at the birth (PC) is a PC=1,82хPS/KR, where PS – the cost of a mink's skin, rub, KR – fertility.

At prime cost of a mink's skin of the 1660th ruble the charge of the main herd will increase in 1,82 times according to a formula therefore charges of 70 males will make 123928,2 rubles taking into account costs of acquisition and use of a preparation (table 5).

Table 5 - Economic indicators when using biostimulator nucleopeptid in feeding of minks male

Indicator	Group
	skilled	control
Charges of 30 males of the main herd of minks, rub	90636	90636
The cost of a preparation per 30 males, rub	256	-
Total expenses, rub	90892	90636
Received puppies per 30 males, animal.	777	750
Prime cost of 1 kit crop, rub.	559,5	686,6

As a result of bigger quantity of the received puppies (4,4 animals on the leapered female) at biostimulator nucleopeptid inclusion to minks males the prime cost of 1 kit crop will be 4,5% lower in comparison with control.

Table 6 - Economic indicators when using biostimulator nucleopeptid in feeding of minks' females

Indicator	g	roup
	The 2nd skilled	control
Charges of 40 females of the minks' main herd, rub.	120848	120848
The cost of a preparation per 40 females, rub.	320	-
Total expenses, rub.	121168	120848
Received puppies per group of females, animals.	168	131
Prime cost of 1 head of a kit crop, rub.	686,6	719,3

Prime cost of 1 head of a kit crop per 40 females of skilled group will be 5% less than prime cost of an kit crop in control group.