A study of alfa feto protein parameter in serum and histological effects of pups liver by SEM in pregnant rats exposed to overdose of aminoglycoside antibiotics
Автор: Mohammed Ali A.R., Abdul-zahra N.I., Amer Kadhim D.
Журнал: Cardiometry @cardiometry
Рубрика: Original research
Статья в выпуске: 28, 2023 года.
Бесплатный доступ
The study has been conducted in Animal House/Faculty of Sciences/University of Kufa between December 2022 and February 2022, fifteen female Albino Rats are used. Gentamicin is aminoglycoside antibiotics widely used during pregnancy for treatment the infections . The present study has been intended to show Alfa Feto Protein parameter in serum and the histological effects of gentamicin on pups liver by SEM in female Albino Rats. The females Rats are randomly divided into three main groups, comprising five rats for each group .The control group is given inta peritoneal injection of physiological normal saline and the second and third group are given inta peritoneal injection of gentamicin doses twenty, forty mg/kg/day respectively for 20 days from the first day to the end of experimental allocated for each female.The rats are sacrificed in 20 th (dpc) to study the Alfa Feto Protein parameter in serum and histological effects of gentamicin on pups liver. The physiological study show negative results in all study groups due to absent of malformations in pups and present histological effects in external architecture structure of pups liver compare with control groups. In conclusion aminoglycoside gentamicin caused histological effects on pupa liver and not absent of malformations in pups due to negative results of Alfa Feto Protein parameter
Pups, pregnant, aminoglycoside, alfa feto protein
Короткий адрес: https://sciup.org/148327109
IDR: 148327109 | DOI: 10.18137/cardiometry.2023.28.4852
Список литературы A study of alfa feto protein parameter in serum and histological effects of pups liver by SEM in pregnant rats exposed to overdose of aminoglycoside antibiotics
- AlTameemi WTM. (2014). Immunohistochemical and molecular detection of Reg3a, Ins1, and Ins2 genes in pancreatic tissues of thymoquinon treated diabetic rats. Ph.D. Thesis. Al-qadisiyah University.
- Arjinajarn, P, et al. (2017). Anthocyanin-rich Riceberry bran extract attenuates gentamicin-induced hepatotoxicity by reducing oxidative stress, inflammation and apoptosis in rats. Biomedicine & pharmacotherapy, 92, 412-420.
- de Montemor Marçal, G, et al. (2021). Association between the consumption of ultra-processed foods and the practice of breast-feeding in children under 2 years of age who are beneficiaries of the conditional cash transfer programme, Bolsa Família. Public Health Nutrition, 24(11), 3313-3321.
- Erhirhie E. Ekene N. and Ajaghaku D. (2014). Guidelines on dosage calculation and stock solution preparation in experimental animals’ studies.J. natu. Sci. R. ISSN 2224-3186.
- Gabant P, et al. (2002). Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility. Proceedings of the National Academy of Sciences, 99(20), 12865-12870.
- Galaly SR, Ahmed OM and Mahmoud AM. (2014). Thymoquinone and curcumin prevent gentamicin- induced liver injury by attenuating oxidative stress, inflammation and apoptosis. J. Physiol. Pharmacol. 65(6): 823-32.
- Hall JE. (2016). Text Book of Medical Physiology. 12th ed. Philadelphia: Saunders Elsevier; 1168 pp.
- Hobbie SN, et al. (2008). Genetic analysis of interactions with eukaryotic rRNA identify the mitoribosome as target in aminoglycoside ototoxicity. Proceedings of the National Academy of Sciences, 105(52), 20888-20893.
- Sigal SH, et al. (1994). Characterization and enrichment of fetal rat hepatoblasts by immunoadsorption (“panning”) and fluorescence‐activated cell sorting. Hepatology, 19(4), 999-1006.
- Survarna SK., Lyaton C. and Bancroft JD. (2018). Bancroft,s Theory and practice of histological technique. Saven edition. Elsevier Limited.,China. 584 pp.
- Tan D. (2017). Anatomy, Physiology, and Husbandry of Laboratory Animals. Fundamentals of Laboratory Animal Science, 129-188.
