Adaptive reactions as a guideline in evaluation of metabolite therapy

Unilateral oophorectomy (UOE) of female rats caused severe stress with an increase in the duration of the non-reproductive period (NRP) - the metestrus and diestrus phases, and a shortening of the reproductive period (RP) - the proestrus and estrus phases in the estrus cycle (a symptom of hormonal deficiency). 5 weeks after UOE, the signs of stress persisted: a decrease in the ratio of lymphocytes to the neutrophilic leukocytes in the peripheral blood and the weight ratio of thymus to the adrenal glands, as well as a decrease in the activity of succinate dehydrogenase, which is determined cytobiochemically in peripheral blood cells. A two-week administration of a succinate-containing composition with B vitamins (SCCV) to control and UOE rats increased the ratio of lymphocytes to neutrophils and weight ratio of the thymus to adrenal glands above the intact level - a sign of the “activation” state according to Garkavi and Kvakina and restored the ratio of NRP/RP. At the same time, a significant increase in SDH activity was found in animal peripheral blood lymphocytes. The study demonstrates the possibility to reverse severe stress state to the “activation” state with the help of metabolic therapy containing the combination of SCC and B-Vit.