Genetic diversity analysis of natural populations and broodstocks of sterlet based on polymorphic ISSR-markers

Genetic diversity of two natural populations of sterlet ( Acipenser ruthenus L., Acipenseridae) was researched: in the lower part of the Sukhona river in Vologda Region and in the middle part of the Kama river in Perm Krai; and also, two sterlet’s broodstocks Saratov branch of FSBSI GosNIORH and fish farm “Topol” in Perm Krai. ISSR (Inter-Simple Sequence Repeats) - method of DNA polymorphism analysis was used for identification of genetic diversity rate while using PCR. 89 ISSR-PCR markers in researched populations of A. ruthenus were identified. The number of amplified ISSR-PCR markers depended on primer and ranged from 5 (primer (CT)8TG) to 15 (primer (CA)6GT), and its size - from 200 (ISSR-9 and X9) to 1500 (X9) bp. In total samples of A. ruthenus the rate of polymorphic loci is high amounted to 0.910, the expected heterozygosity is 0.296 and the number of effective alleles is - 1.518. The index of genetic diversity is higher in population from Kama river (HE = 0.224; ne=1.380) and lower in broodstock from “Topol” fish farm (HE= 0.170; ne =1,290). The highest number of rare ISSR-PCR markers were identified in natural population from Kama river (R=3), while in broodstock from “Topol” fish farm no rare ISSR-PCR markers were identified. The group of natural populations is more genetically heterogeneous in comparison with the group of broodstocks. Genetic structure of two natural populations (Gst=0.297) and two broodstocks of A. ruthenus (Gst=0.281) was established. Recommendations for genetic diversity data using of the studied populations and broodstocks have been provided for the gene pool conservation.