Antimutagenic activity of probiotic cultures and microbial consortia

The natural origin of probiotic cultures, and the availability of information about their antimutagenic activity, determined the interest in studying the antimutagenic properties of certain strains of probiotics and their consortia, and in the future, their practical application. In this work, strains of propionic acid bacteria, Acidophilus bacillus and kefir fungal starter culture were used as independent test objects, and in a certain combination. The bacterial Ames test was used to study the antimutagenic properties of cultures and consortia. As a result of the conducted studies, high antimutagenic properties have been established. The degree of inhibition for L. acidophilus is 50.9 %, for the Propionibacterium freundenreichii strain subsp. shermanii AC-2503 - 63.4 %. The maximum level of inhibition was observed in the Propionibacterium freudenreichii strain SH-85 - 65.6 %. Kefir fungal starter culture, as a natural symbiotic cluster, certainly shows a significant level of antimutagenic activity - 39 %. It was found that consortia have higher antimutagenic activity compared to monocultures. The degree of mutagen inhibition by the consortium of L. acidophilus and Propionibacterium freudenreichii SH-85 cultures is of the greatest importance and is 70.5 %. Consortia based on kefir culture and Propionibacterium freundenreichii subsp. shermanii AC-2503, kefir culture and Propionibacterium freudenreichii SH-85 have antimutagenic activity values of 69.3 % and 67.5 %, respectively. Thus, propionic acid bacteria Propionibacterium freundenreichii subsp. shermanii AC-2503, Propionibacterium freudenreichii SH-85, L. acidophilus, kefir fungal starter culture and consortia created on the basis of these cultures are valuable sources of antimutagens and can be recommended for the production of bacterial concentrates and bio-products.