Approbation of a developed method for determining antibiotics of a tetracycline group in honey by method of HPLC in RT

An algorithm is developed for sample preparation and selected conditions chromatographically the simultaneous determination of antibiotics (tetracycline hydrochloride, oxytetracycline, chlortetracycline) in honey at the level of the MRL (0.01 mg/kg) according to SanPiN 2.3.2.1078-01 in artificial condition, using a liquid chromatograph "Agilent1260 Infiniti", equipped with a thermostat column, gradient pump and UV detector. According to the results of the studies it was found that the most optimal condition for the simultaneous determination of antibiotics (oxytetracycline, tetracycline hydrochloride, chlortetracycline) in honey by HPLC with UV detection was the wavelength of 254 nm, flow rate - 0.5 ml/min, column temperature 250C. Under these conditions, the retention time of antibiotics, which was for oxytetracycline - 4,069 min, tetracycline hydrochloride - 4,331 min, chlortetracycline - 4,642 min. The developed HPLC method of simultaneous determination of tetracycline antibiotics in honey was tested on bee products from 5 districts of the Republic of Tatarstan. The obtained results exclude the possibility of honey contamination with antimicrobial agents